

B-SC in Biotechnology at RK University


Rajkot, Gujarat
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About the Specialization
What is Biotechnology at RK University Rajkot?
This Biotechnology program at RK University focuses on the comprehensive study of biological processes and their application in various industries. With a strong emphasis on practical skills and research, the curriculum is designed to meet the evolving demands of the Indian biotechnology sector, encompassing areas from medical and agricultural to industrial applications. The program aims to cultivate innovative thinkers for impactful contributions.
Who Should Apply?
This program is ideal for 10+2 Science graduates with a keen interest in biological sciences and a desire to apply scientific principles to solve real-world problems. It''''s also suitable for individuals aspiring to pursue careers in research, healthcare, agriculture, pharmaceuticals, or entrepreneurial ventures within the rapidly growing Indian biotech industry.
Why Choose This Course?
Graduates of this program can expect diverse career paths in India''''s booming biotechnology sector, including roles as research associates, quality control analysts, production executives, or bioinformatics specialists. Entry-level salaries typically range from INR 2.5 to 4.5 lakhs annually, with significant growth trajectories for experienced professionals in R&D or management roles. The program also lays a strong foundation for higher studies like M.Sc. or Ph.D.

Student Success Practices
Foundation Stage
Master Core Biological Concepts- (Semester 1-2)
Focus on building a strong foundation in subjects like Cell Biology, Biochemistry, and Microbiology. Utilize textbooks, online lectures (NPTEL, Coursera), and peer study groups to clarify concepts. Regular revision and problem-solving are crucial for academic excellence.
Tools & Resources
NPTEL courses, Khan Academy Biology, Peer study groups, RK University Library Resources
Career Connection
A robust understanding of fundamentals is essential for advanced biotechnology subjects and forms the basis for research and industry applications.
Develop Laboratory Proficiency- (Semester 1-2)
Actively participate in all practical sessions, understanding the ''''why'''' behind each step. Seek opportunities to spend extra time in labs (if permitted) or volunteer for department projects to gain hands-on experience with equipment and techniques. Maintain detailed lab notebooks.
Tools & Resources
Laboratory manuals, YouTube tutorials for specific techniques, Lab supervisors/faculty
Career Connection
Strong practical skills are highly valued by employers in biotech R&D, quality control, and production roles, making you job-ready.
Enhance Scientific Communication- (Semester 1-2)
Practice presenting scientific concepts clearly, both orally and in writing. Participate in departmental seminars, prepare comprehensive lab reports, and seek feedback on your communication style. Join relevant clubs for presentation opportunities.
Tools & Resources
Grammarly, Microsoft PowerPoint, Departmental seminars, Public speaking clubs
Career Connection
Effective communication is vital for conveying research findings, collaborating in teams, and writing successful grant proposals or technical reports in any scientific career.
Intermediate Stage
Engage in Mini-Projects and Internships- (Semester 3-5 (especially during summer breaks))
Seek out faculty-led mini-projects or pursue short-term internships during breaks. This provides exposure to real-world research problems, advanced techniques, and industry environments. Look for opportunities in local biotech companies or research institutes in Gujarat.
Tools & Resources
Faculty advisors, Industry contacts through university network, Online internship portals (Internshala)
Career Connection
Practical industry/research exposure differentiates your resume, builds professional networks, and can lead to pre-placement offers or stronger job prospects.
Specialize through Electives & Certifications- (Semester 4-5)
Carefully choose elective subjects that align with your career interests (e.g., Medical, Industrial, Agricultural Biotech). Supplement your studies with online certifications in niche areas like Bioinformatics, Molecular Diagnostics, or Fermentation Technology.
Tools & Resources
Coursera, edX, NPTEL, Biotech training institutes in Gujarat
Career Connection
Specialized knowledge and certifications make you a more targeted candidate for specific roles and can increase your earning potential.
Participate in Competitions and Workshops- (Semester 3-5)
Engage in biotech quizzes, poster presentations, paper presentations, and workshops organized by the university or external bodies. This sharpens your knowledge, critical thinking, and networking skills. Consider national level competitions like BioQuiz.
Tools & Resources
University event calendar, Biotech associations in India, Departmental notices
Career Connection
Such participation builds confidence, expands your professional network, and demonstrates initiative, making you a more attractive candidate for higher studies or jobs.
Advanced Stage
Undertake a Comprehensive Research Project- (Semester 4-6 (with major focus in Semester 6))
Utilize the final year project (BTY609) as a major opportunity for in-depth research. Choose a topic that excites you and aligns with industry trends. Dedicate significant effort to design experiments, collect robust data, and produce a high-quality dissertation.
Tools & Resources
Faculty mentors, Advanced lab facilities, Literature databases (PubMed, Scopus), Statistical software
Career Connection
A strong final year project is a powerful portfolio piece for research-oriented careers, M.Sc./Ph.D. admissions, and showcases problem-solving abilities to employers.
Network and Attend Industry Events- (Semester 5-6)
Actively network with professionals, alumni, and recruiters through LinkedIn, career fairs, and industry seminars. Attend conferences or workshops in your area of interest to stay updated on emerging technologies and market demands in India.
Tools & Resources
LinkedIn, University alumni network, Industry conferences (e.g., BIOASIA), Career services cell
Career Connection
Networking opens doors to hidden job opportunities, mentorship, and insights into career pathways, significantly aiding placement.
Prepare for Placements and Higher Education- (Semester 5-6)
Start preparing for interviews, aptitude tests, and group discussions early. Develop a strong resume and cover letter. For higher education, prepare for entrance exams (like GATE-BT, BET-JRF, or GRE/TOEFL for abroad) and craft compelling statements of purpose. Utilize the university''''s placement cell extensively.
Tools & Resources
RKU Placement Cell, Online aptitude platforms, Interview preparation guides, Career counselors
Career Connection
Thorough preparation ensures you successfully transition into your desired career path, whether it''''s immediate employment or advanced academic pursuits.
Program Structure and Curriculum
Eligibility:
- Passed 10+2 (Science Stream) or its equivalent examination with Physics, Chemistry, Biology/Mathematics/Biotechnology as mandatory subjects from a recognized Board/University with a minimum 45% for general category and 40% for SC/ST/SEBC category.
Duration: 3 years (6 semesters)
Credits: 153 Credits
Assessment: Internal: 30%, External: 70%
Semester-wise Curriculum Table
Semester 1
| Subject Code | Subject Name | Subject Type | Credits | Key Topics |
|---|---|---|---|---|
| BTY101 | Introduction to Biotechnology | Core | 3 | Introduction to Biotechnology and its Branches, Scope and Importance of Biotechnology, Basic Concepts of Cells, DNA, Proteins, Ethical Issues in Biotechnology, Historical Development of Biotechnology |
| BTY102 | General Microbiology | Core | 3 | History and Scope of Microbiology, Classification and Diversity of Microbes, Structure and Function of Microbial Cells, Microbial Growth and Nutrition, Control of Microorganisms (Sterilization, Disinfection) |
| BTY103 | Cell Biology | Core | 3 | Prokaryotic and Eukaryotic Cell Structure, Cell Organelles and their Functions, Cell Division: Mitosis and Meiosis, Cell Cycle Regulation, Cell Signaling Mechanisms |
| BTY104 | Biochemistry | Core | 3 | Biomolecules: Carbohydrates, Proteins, Lipids, Nucleic Acids: Structure and Types, Enzymes: Classification, Mechanism, Kinetics, Metabolic Pathways: Glycolysis, Krebs Cycle, Bioenergetics |
| BTY105 | Bioanalytical Techniques | Core | 3 | pH and Buffers, Electrolytes, Spectroscopic Techniques (UV-Vis, Fluorescence), Chromatography (Paper, Thin Layer, Column), Electrophoresis (Agarose, SDS-PAGE), Centrifugation and Microscopy |
| BTY106 | Computer Applications in Biology | Core | 2 | Introduction to Computers and Operating Systems, MS Office Suite (Word, Excel, PowerPoint), Internet and Web Browsing for Biological Data, Introduction to Biological Databases, Basic Data Analysis and Presentation |
| BTY107 (P) | General Microbiology Practical | Lab | 2 | Microscope handling and observation, Staining techniques (Gram, Spore), Preparation of culture media, Isolation and culturing of microbes, Antibiotic sensitivity testing |
| BTY108 (P) | Cell Biology Practical | Lab | 2 | Observation of plant and animal cells, Mitosis and Meiosis observation in onion root/grasshopper testis, Cell counting using hemocytometer, Staining of cell organelles, Osmosis and diffusion experiments |
| BTY109 (P) | Biochemistry Practical | Lab | 2 | Qualitative tests for carbohydrates, proteins, lipids, Estimation of amino acids and proteins, Enzyme activity assay, Effect of pH and temperature on enzyme activity, Preparation of standard solutions |
| BTY110 (P) | Bioanalytical Techniques Practical | Lab | 2 | pH measurement and buffer preparation, Use of spectrophotometer for quantification, Paper and Thin Layer Chromatography, Centrifugation techniques, Electrophoresis demonstration |
Semester 2
| Subject Code | Subject Name | Subject Type | Credits | Key Topics |
|---|---|---|---|---|
| BTY201 | Molecular Biology | Core | 3 | DNA Structure and Replication, RNA Structure and Transcription, Protein Synthesis (Translation), Gene Expression Regulation (Prokaryotic & Eukaryotic), Mutations and DNA Repair Mechanisms |
| BTY202 | Immunology | Core | 3 | Components of Immune System (Organs, Cells), Antigens and Antibodies, Humoral and Cell-Mediated Immunity, Hypersensitivity and Autoimmunity, Vaccines and Immunization |
| BTY203 | Genetics | Core | 3 | Mendelian Principles of Inheritance, Extensions of Mendelian Genetics (Gene Interaction, Multiple Alleles), Linkage and Crossing Over, Chromosomal Aberrations, Population Genetics and Human Genetics |
| BTY204 | Biostatistics | Core | 3 | Introduction to Biostatistics and Data Types, Measures of Central Tendency and Dispersion, Probability and Probability Distributions, Hypothesis Testing (t-test, Chi-square), Correlation and Regression |
| BTY205 | Environmental Biotechnology | Core | 3 | Environmental Pollution and its Types, Bioremediation of Pollutants (Oil, Heavy Metals), Waste Management (Solid, Liquid Waste), Biofuels and Renewable Energy, Global Environmental Issues |
| BTY206 (P) | Molecular Biology Practical | Lab | 2 | DNA isolation from bacterial/plant cells, RNA isolation, Agarose Gel Electrophoresis of DNA/RNA, Polymerase Chain Reaction (PCR) demonstration, Quantification of DNA/RNA |
| BTY207 (P) | Immunology Practical | Lab | 2 | Blood grouping and Rh typing, ELISA (Enzyme-Linked Immunosorbent Assay), Radial Immunodiffusion, Precipitation and Agglutination reactions, Leucocyte count |
| BTY208 (P) | Genetics Practical | Lab | 2 | Preparation of human karyotype, Study of Mendelian ratios in Drosophila/Maize, Pedigree analysis of genetic traits, Solving genetics problems, Chromosomal aberration identification |
| BTY209 (P) | Biostatistics Practical | Lab | 2 | Data presentation using graphs and tables, Calculation of mean, median, mode, standard deviation, t-test for comparison of means, Chi-square test for association, Using statistical software (Excel/R for basic analysis) |
| BTY210 (P) | Environmental Biotechnology Practical | Lab | 2 | Analysis of water quality parameters (pH, DO, BOD, COD), Microbial degradation of pollutants, Isolation of microbes from contaminated sites, Biogas production demonstration, Composting techniques |
Semester 3
| Subject Code | Subject Name | Subject Type | Credits | Key Topics |
|---|---|---|---|---|
| BTY301 | Genetic Engineering | Core | 3 | Principles of Recombinant DNA Technology, Cloning Vectors (Plasmids, Phages, Cosmids), Restriction Enzymes and DNA Ligase, Gene Transfer Methods (Transformation, Transfection), Gene Editing Technologies (CRISPR-Cas9) |
| BTY302 | Animal Cell Culture | Core | 3 | Principles and Sterilization Techniques, Cell Culture Media and Supplements, Primary Cell Culture and Cell Lines, Cryopreservation and Cell Banking, Applications of Animal Cell Culture |
| BTY303 | Plant Biotechnology | Core | 3 | Principles of Plant Tissue Culture, Micropropagation and Organogenesis, Somatic Hybridization and Somaclonal Variation, Transgenic Plants and their Applications, Molecular Markers in Plant Breeding |
| BTY304 | Fermentation Technology | Core | 3 | Fermenter Design and Operation, Microbial Growth Kinetics, Upstream and Downstream Processing, Production of Industrial Enzymes, Bioproducts (Antibiotics, Organic Acids) |
| BTY305 | Bioinformatics | Core | 3 | Introduction to Bioinformatics and Biological Databases, Sequence Alignment (BLAST, FASTA), Phylogenetic Analysis, Protein Structure Prediction, Introduction to Drug Designing |
| BTY306 (P) | Genetic Engineering Practical | Lab | 2 | Isolation of plasmid DNA, Restriction digestion of DNA, Ligation of DNA fragments, Bacterial transformation, Gel electrophoresis of digested DNA |
| BTY307 (P) | Animal Cell Culture Practical | Lab | 2 | Preparation of animal cell culture media, Sterilization techniques for cell culture, Maintenance of cell lines (passaging), Cryopreservation of animal cells, Cell viability assay (Trypan blue exclusion) |
| BTY308 (P) | Plant Biotechnology Practical | Lab | 2 | Preparation of plant tissue culture media, Sterilization of explants, Initiation of callus culture, Micropropagation of selected plants, Protoplast isolation |
| BTY309 (P) | Fermentation Technology Practical | Lab | 2 | Construction and operation of a laboratory fermenter, Estimation of microbial growth kinetics, Production of alcohol by yeast fermentation, Isolation of an industrial enzyme, Quantification of fermented products |
| BTY310 (P) | Bioinformatics Practical | Lab | 2 | Sequence retrieval from NCBI databases, Performing BLAST searches for sequence similarity, Multiple sequence alignment using ClustalW, Phylogenetic tree construction, Protein secondary structure prediction |
Semester 4
| Subject Code | Subject Name | Subject Type | Credits | Key Topics |
|---|---|---|---|---|
| BTY401 | Genomics & Proteomics | Core | 3 | Genome Sequencing Technologies (Next-Gen Sequencing), Structural and Functional Genomics, Proteomics: Protein Identification and Characterization, Mass Spectrometry in Proteomics, Gene and Protein Microarrays |
| BTY402 | Industrial Biotechnology | Core | 3 | Enzyme Technology and Biocatalysis, Production of Industrial Chemicals, Food and Beverage Biotechnology, Pharmaceutical Production, Biorefineries and Biofuels |
| BTY403 | Nano-biotechnology | Core | 3 | Introduction to Nanomaterials and Nanoscale Phenomena, Synthesis of Nanoparticles (Biological and Chemical), Characterization Techniques for Nanomaterials, Applications in Medicine (Drug Delivery, Diagnostics), Nanobiotechnology in Agriculture and Environment |
| BTY404 | Food Biotechnology | Core | 3 | Food Processing and Preservation Techniques, Fermented Foods and Beverages, Food Additives and Ingredients, Food Safety and Quality Control, Genetically Modified Foods |
| BTY405 | Intellectual Property Rights in Biotechnology | Core | 3 | Introduction to IPR (Patents, Trademarks, Copyrights), Patentability of Biotechnological Inventions, Patent Filing and Infringement, Ethical Issues in Biotechnology IP, Biodiversity and Traditional Knowledge Protection |
| BTY406 (P) | Genomics & Proteomics Practical | Lab | 2 | DNA fingerprinting techniques, SDS-PAGE for protein separation, Western blotting demonstration, Bioinformatics tools for genomics data analysis, Basic image analysis of gels/blots |
| BTY407 (P) | Industrial Biotechnology Practical | Lab | 2 | Enzyme immobilization techniques, Bioconversion of substrates by microbes, Quantification of industrial products (e.g., ethanol), Isolation of industrially important microbes, Study of bioreactor components |
| BTY408 (P) | Nano-biotechnology Practical | Lab | 2 | Synthesis of metal nanoparticles (e.g., silver, gold), Characterization of nanoparticles (UV-Vis spectroscopy), Application of nanoparticles in biosensing, Microbial synthesis of nanoparticles, Antimicrobial activity of nanoparticles |
| BTY409 (P) | Food Biotechnology Practical | Lab | 2 | Microbiological analysis of food samples, Production of fermented food products (yogurt, bread), Detection of food adulterants, Quality assessment of food products, Isolation of probiotics |
| BTY410 (Project) | Project / Dissertation | Project | 4 | Identification of a research problem, Literature review and experimental design, Data collection and analysis, Scientific report writing, Oral presentation of findings |
Semester 5
| Subject Code | Subject Name | Subject Type | Credits | Key Topics |
|---|---|---|---|---|
| BTY501 | Medical Biotechnology | Core | 3 | Gene Therapy and its Applications, Stem Cell Technology and Regenerative Medicine, Molecular Diagnostics (PCR, Microarrays), Drug Discovery and Development, Vaccine Development and Delivery |
| BTY502 | Agricultural Biotechnology | Core | 3 | Crop Improvement through Biotechnology, Biofertilizers and Biopesticides, Stress Tolerance in Plants (Abiotic & Biotic), Transgenic Crops and their Benefits, Genomics and Proteomics in Agriculture |
| BTY503 | Environmental Impact Assessment (EIA) | Core | 3 | Introduction to EIA and its Principles, Stages and Methodologies of EIA, Environmental Policies and Regulations, Case Studies of EIA in India, Risk Assessment and Management |
| BTY504 | Entrepreneurship in Biotechnology | Core | 3 | Business Planning and Market Analysis, Funding Sources for Biotech Startups, Regulatory Affairs and IP for Biotech Ventures, Innovation and Technology Transfer, Case Studies of Successful Biotech Startups |
| BTY505 (A) | Bioprocess Engineering | Elective | 3 | Bioreactor Design and Operation, Mass and Heat Transfer in Bioreactors, Sterilization Techniques for Bioprocesses, Process Control and Instrumentation, Downstream Processing Principles |
| BTY505 (B) | Marine Biotechnology | Elective | 3 | Diversity of Marine Organisms, Bioactive Compounds from Marine Sources, Aquaculture and Fisheries Biotechnology, Marine Bioremediation, Algal Biotechnology |
| BTY505 (C) | Pharmaceutical Biotechnology | Elective | 3 | Drug Discovery and Development Process, Recombinant Biopharmaceuticals, Clinical Trials and Regulatory Approval, Good Manufacturing Practices (GMP), Quality Control in Pharma Industry |
| BTY506 (P) | Medical Biotechnology Practical | Lab | 2 | Maintenance of human cell lines, Molecular diagnostics for pathogens (e.g., LAMP), ELISA for disease markers, Drug efficacy testing on cell cultures, Gene expression analysis (RT-PCR demonstration) |
| BTY507 (P) | Agricultural Biotechnology Practical | Lab | 2 | Transformation of plants (e.g., Agrobacterium-mediated), Detection of transgenes in plants, Isolation of biofertilizer microbes, Study of stress responses in plants, Molecular marker application demonstration |
| BTY508 (P) | Environmental Impact Assessment Practical | Lab | 2 | Case study analysis of industrial projects, Preparation of a mini EIA report, Field visit to an industrial site for environmental assessment, Data collection on environmental parameters, Risk assessment methodology application |
| BTY509 (P) | Bioprocess Engineering Practical / Marine Biotechnology Practical / Pharmaceutical Biotechnology Practical | Lab | 2 | Practical experiments based on the chosen elective subject., Specific hands-on experience relevant to the elective., Example: Bioreactor scale-up calculation (Bioprocess Engg.), Example: Isolation of marine microbes (Marine Biotech), Example: Formulation of a drug product (Pharma Biotech) |
Semester 6
| Subject Code | Subject Name | Subject Type | Credits | Key Topics |
|---|---|---|---|---|
| BTY601 | Recombinant Therapeutics | Core | 3 | Production of Recombinant Proteins (Insulin, Growth Hormone), Monoclonal Antibodies and their Applications, Recombinant Vaccines and Diagnostics, Gene Therapy Products, Biosimilars and Biologics |
| BTY602 | Biosafety and Bioethics | Core | 3 | Biosafety Levels and Containment Facilities, Regulations for Genetically Modified Organisms (GMOs), Ethical Aspects of Genetic Engineering, Animal Welfare in Research, Public Perception and Acceptance of Biotechnology |
| BTY603 | Quality Control & Regulatory Affairs | Core | 3 | Good Laboratory Practices (GLP) and Good Manufacturing Practices (GMP), Quality Assurance and Quality Control in Biotech Industry, Regulatory Agencies (FDA, EMA, CDSCO), Documentation and Auditing, Drug Approval Process |
| BTY604 (A) | Tissue Engineering & Regenerative Medicine | Elective | 3 | Principles of Tissue Engineering, Scaffolds and Biomaterials, Stem Cell Biology and Therapy, Organ Regeneration and Bioprinting, Clinical Applications of Regenerative Medicine |
| BTY604 (B) | Computational Biology | Elective | 3 | Algorithms in Biology, Systems Biology and Network Analysis, Molecular Modeling and Drug Design, Genomic Data Mining and Analysis, Next-Generation Sequencing Data Analysis |
| BTY604 (C) | Food Safety & Quality Management | Elective | 3 | Foodborne Pathogens and Toxins, HACCP and ISO Standards in Food Industry, Food Adulteration and Detection, Risk Assessment in Food Safety, Traceability and Supply Chain Management |
| BTY605 (P) | Recombinant Therapeutics Practical | Lab | 2 | Purification of a recombinant protein, SDS-PAGE analysis of protein purity, ELISA for quantification of recombinant protein, Cell-based assays for therapeutic molecules, Immunoblotting techniques |
| BTY606 (P) | Biosafety and Bioethics Practical | Lab | 2 | Risk assessment for laboratory procedures, Operation of biosafety cabinets, Preparation of Standard Operating Procedures (SOPs), Discussion on ethical dilemmas in biotechnology, Disposal of biohazardous waste |
| BTY607 (P) | Quality Control & Regulatory Affairs Practical | Lab | 2 | Quality testing of a biotech product, Preparation of documentation for regulatory submission, Mock audit of a quality system, Validation of an analytical method, Calibration of laboratory equipment |
| BTY608 (P) | Tissue Engineering & Regenerative Medicine Practical / Computational Biology Practical / Food Safety & Quality Management Practical | Lab | 2 | Practical experiments based on the chosen elective subject., Specific hands-on experience relevant to the elective., Example: Cell seeding on scaffolds (Tissue Engg.), Example: Molecular docking (Computational Biology), Example: HACCP plan development (Food Safety) |
| BTY609 (Project) | Project | Project | 8 | In-depth research and experimental work, Advanced data analysis and interpretation, Comprehensive scientific thesis writing, Presentation of research outcomes to an expert panel, Problem-solving and critical thinking application |




